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Reagents and tools table
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Reagents and tools table

Journal: EMBO Reports

Article Title: miRNA-target complementarity in cnidarians resembles its counterpart in plants

doi: 10.1038/s44319-024-00350-z

Figure Lengend Snippet: Reagents and tools table

Article Snippet: In vitro transcription was conducted with HighYield T7 Cap 1 AG (3‘-OMe) mRNA Synthesis Kit (m5CTP) (Jena Bioscience, Germany) using 800 ng of amplified template followed by Turbo DNase treatment (Thermo Fisher Scientific) by incubation with 1 µl of DNase for 30 min at 37 °C twice sequentially.

Techniques: Sequencing, shRNA, SYBR Green Assay, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Magnetic Beads, Multiplex Assay, Sensitive Assay, Software, Imaging, Spectrophotometry, Real-time Polymerase Chain Reaction